An frt-flanked neo cassette with a 5' loxP site was inserted upstream of exon 2 and an additional loxP site was inserted downstream of exon 2. Germ-line, flp-mediated recombination was used to remove the neo cassette. (J:133262)
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An frt-flanked neo cassette with a 5' loxP site was inserted upstream of exon 2 and an additional loxP site was inserted downstream of exon 2. Germ-line, flp-mediated recombination was used to remove the neo cassette. (J:133262)