Ifi208^{Tg(Cspg4-cre)1Akik}

The transgene construct consisted of the 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), which was modified by inserting an nls-cre coding sequence followed by a rabbit beta-globin polyA signal into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2 gene and to allow translation from the first ATG in the cre sequence. Cre expression is detected only in Cspg4-expressing cells at P1, P14 and P60 and not in Gfap +ve astrocytes or APC +ve oligodendrocytes. Activity is also detected in male germ cells. Line 1 inserted into an intron of the gene at 173692115 (Build GRCm38/mm10). Founder line 1 has a copy number of 1-3. (J:129201, J:185534)