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A full-length, 0.8-kb cDNA encoding the mouse MAFK protein with a N-terminal FLAG epitope was inserted into the VA CD2 expression vector between, 5', the promoter, ATG-free mutant first exon and first intron of the human CD2 gene and 3', also from the CD2 gene, ~500 bp of 3' untranslated and flanking DNA including two polyadenylation signals and the 3' locus control region. A lacZ reporter gene was shown to be expressed from this vector in mature peripheral CD4+CD8- and CD4-CD8+ SP T lymphocytes and in peripheral CD4-CD8- DN T cells. This line was estimated by densitometric analysis to carry 6 copies of the transgene. (J:105228, J:128658)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
