Exons 4 through 12 were replaced with a genomic segment containing exons 1 through 4 with a GFP cassette inserted into intron 3 and a neo cassette inserted into exon 4. Expression of the GFP cassette was confirmed by fluorescence microscopy. Transcriptional efficiency of the allele is reduced compared to wild-type. (J:132703)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count