Tg(Hoxc11/lacZ)62D9Awg

The transgene was made from a 10-kb mouse genomic DNA fragment, derived from the CD-1 genomic cosmid clone cos2W3a, containing the Hoxc11 coding region with 2 kb of upstream and 5 kb of downstream flanking DNA. The E. coli lacZ coding sequence was inserted in-frame at an Eag1 restriction site in exon 1, replacing an internal Eag1 DNA fragment. Expression of beta-galactosidase from the transgene in 11.5-13.5 dpc embryos, detected by Xgal staining, recapitulates that of endogenous HOXC11 in the hindlimb and posterior proximal forelimb. (J:61447)