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A GFP-neo cassette with sequence encoding a 14 amino acid linker was inserted in frame into exon 2 replacing most of it. Only the first 4 amino acids of exon 2, the first coding exon, are expressed. The presence of the fusion transcript and protein was confirmed by RT-PCR and immunohistochemistry, respectively. The absence of the endogenous transcript was confirmed by RT-PCR. This is a null allele. (J:132129)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
