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A targeting vector was designed to introduce a loxP-flanked neomycin-resistance cassette in reverse transcriptional orientation to intron 17 and an additional loxP site to intron 18. Exon 18 encodes the transmembrane domain of the gene (also called CD148). The targeting construct was transfected into E14.1TG3B1 129P2/OlaHsd-derived embryonic stem (ES) cells. Targeted animals were bred with BALB/c-background CMV-Cre transgenic female mice. Progeny lacking the neomycin cassette, but retaining exon 18 flanked by loxP sites were bred to remove the CMV-Cre transgene. (J:131736)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
