Chimeric males with the floxed region were crossed to cre-expressing females and exons 1 through 5 were removed in the germ line. The absence of transcript was confirmed by northern blot analysis on E10.5 embryos extracts. (J:131268)
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Chimeric males with the floxed region were crossed to cre-expressing females and exons 1 through 5 were removed in the germ line. The absence of transcript was confirmed by northern blot analysis on E10.5 embryos extracts. (J:131268)