An frt-flank neo cassette with a 3' lox P site was inserted upstream of the open reading frame and an additional lox P site was inserted downstream of the open reading frame. Flp-mediated recombination was used to remove the neo cassette. (J:129742)
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An frt-flank neo cassette with a 3' lox P site was inserted upstream of the open reading frame and an additional lox P site was inserted downstream of the open reading frame. Flp-mediated recombination was used to remove the neo cassette. (J:129742)