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A floxed neo cassette was inserted into intron 14 and additional loxP site was inserted into intron 15. Cre mediated recombination was used to remove the neo cassette and exon 15. The protein product lacks the last 24 amino acids that contains the ATM binding domain. The presence of a shortened protein product was confirmed by western blot analysis on extracts from mouse embryonic fibroblasts. (J:129762)