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The gene was disrupted by insertion into exon 2, which encodes the DNA-binding TEA domain of the protein, of two functional cassettes: 1) a loxP-flanked Pgk-neo-poly(A) cassette, followed by 2) a cDNA encoding the beta-geo (E. coli beta-galactosidase/neomycin resistance) fusion protein flanked 5' by an internal ribosomal entry site (IRES) and 3' by a polyadenylation signal. Reverse transcription- (RT-) PCR using primers in exons 5 and 6 detects no transcripts from the mutant allele in homozygous E3.5 embryos. Whereas immunohistochemical analysis with antisera to TEAD4 stains all cell nuclei of 34-36-cell wild-type embryos, mutant embryos produce no signal. (J:130047)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
