An frt-flanked neo cassette with a 5' loxP site was inserted downstream of exon 3. An additional loxP site was inserted upstream of exon 3. Flp-mediated recombination was used to remove the neo cassette leaving a floxed exon 3. (J:129075)
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An frt-flanked neo cassette with a 5' loxP site was inserted downstream of exon 3. An additional loxP site was inserted upstream of exon 3. Flp-mediated recombination was used to remove the neo cassette leaving a floxed exon 3. (J:129075)