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A loxP-flanked neomycin selection cassette and an IRES-GFP reporter gene were inserted immediately downstream of the stop codon and upstream of the endogenous polyadenylation signal sequence. Targeting took place in ES cells homozygous for the Tg(Prm-cre)70Og transgene, which expresses Cre recombinase in male germ cells. Cre-mediated excision subsequently removed the neomycin cassette upon transmission from male chimeric mice. Reporter gene expression was confirmed by immunohistochemistry on intestine and kidney from homozygotes. (J:128682)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
