A floxed neo cassette was inserted upstream of exon 5 with an additional loxP site inserted downstream of exon 5. Cre-mediated recombination using a transiently transfected pMC-Cre vector was used to remove the neo cassette. (J:128523)
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A floxed neo cassette was inserted upstream of exon 5 with an additional loxP site inserted downstream of exon 5. Cre-mediated recombination using a transiently transfected pMC-Cre vector was used to remove the neo cassette. (J:128523)