A loxP site was inserted 300 bp upstream of the translation start site and a second loxP site was inserted along with an Frt flanked neo cassette immediately 3' of the coding region. The neo cassette was removed by in vivo Flp-mediated recombination leaving the entire coding region flanked with loxP sites. (J:128312)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count