Epo^{Tg(TAg/Epo)134.3LCPjr}

This transgene contains the wild-type allele of the SV40 large T antigen (TAg) gene fused to the mouse Epo gene isolated from a BALB/c library. In this founder line, a single copy of the transgene replaced one copy of the endogenous Epo gene. This recombination event was confirmed by restriction digest mapping and Southern blotting. Sv40 T antigen was detected in the interstitial cells of the kidney. Epo transgene expression in the kidney is reduced compared to endogenous Epo gene expression as determined by RNAse protection assay.