A floxed cassette containing both Neo and TK selection markers was introduced into the second intron and a third loxP site into intron 4 of the gene. After transient Cre expression, clones were selected that had the selection cassette removed while leaving exons 3 and 4 flanked by loxP sites. PCR confirmed insertion of loxP sites. (J:127458)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count