The entire coding sequence, contained within exon 2, was replaced with a PGK-neo cassette. The absence of transcript and protein production was confirmed by RT-PCR and western blot analysis, respectively, using atria and ventricle tissue. (J:127150)
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The entire coding sequence, contained within exon 2, was replaced with a PGK-neo cassette. The absence of transcript and protein production was confirmed by RT-PCR and western blot analysis, respectively, using atria and ventricle tissue. (J:127150)