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A targeting vector in which the promoter and the first exon of the gene are flanked by loxP sites was constructed. The upstream loxP site with a diagnostic XmaCI restriction site was placed into a BSMI site, and the downstream loxP site was cloned into the SexA1 site. A frt-flanked neomycin cassette was placed between exon 1 and the downstream loxP site. Recombination was confirmed through PCR and Southern blot analysis. (J:125912)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
