Tg(CAG-Ub*G76V/GFP)1Dant

The UbG76V-GFP transgene was designed with a chicken beta-actin promoter (and cytomegalovirus (CMV) immediate early enhancer) upstream of a green fluorescent protein (GFP) fused to a mutant ubiquitin moiety (UbG76V). Although transcripts of the UbG76V-GFP fusion gene are detected in all tissues examined, the G76V substitution prevents removal of the ubiquitin moiety leading to efficient ubiquitination and proteasomal degradation of the fusion protein (and thus no fluorescence) in these tissues. Line 1 with high expression was maintained by backcrosses to C57BL/6N mice. The high expressing line (UbG76V-GFP/1) has very low, but detectable, fluorescence expression in brain, spinal cord, and exocrine pancreatic tissue prior to administration of proteasome inhibitors. (J:127085, J:127087)