An FRT flanked neo cassette with a 3' loxP site was inserted between exon 3 and 4, and an additional loxP site was inserted between exons 6 and 7. Flp-mediate recombination to remove the neo cassette was accomplished using Tg(ACTFLPe)9205Dym. Insertion was confirmed by Southern blot analysis and PCR genotyping. (J:126339)
Basic Information
129S1/Sv-Oca2+ Tyr+ Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count