A loxP site was placed 335 bp upstream of exon 2. A second loxP site along with a Frt-flanked PGK-neomycin selection cassette was placed within exon 2, 550 bp downstream of the stop codon and deleting the final 66 bp of the exon. Expression of the targeted locus was unchanged in homozygous mice as determined by quantitative PCR. (J:126375)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129 x C57BL/6)F1
Targeted
Insertion
--
1
2
12

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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