Tg(Thy1-Brainbow1.1)MLich

The Thy1-Brainbow 1.1 transgene was designed with the mouse Thy1 regulatory elements surrounding four fluorescent proteins (orange (OFP), red (RFP), enhanced yellow (EYFP), and cyan (CFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.1 coding region contained (from 5' to 3') a LoxN site, Lox2272 site, LoxP site, Kusabira-Orange OFP (kOFP; with polyA sequence), LoxN site, mCherry RFP (with membrane tethering palmitoylation sequence and polyA sequence), Lox2272 site, monomeric EYFP (mYFPA206K; with membrane tethering palmitoylation sequence and polyA sequence), LoxP site, mCerulean CFP (with membrane tethering palmitoylation sequence and 5? FRT site and polyA sequence). Kusabira-Orange (KO or kOFP) is a true OFP with an artificial amino acid sequence attached to the N-terminus of the protein allowing bright orange fluorescence in cells. The mCherry RFP is an optimized DsRed/mRFP1 fluorescent protein variant with a combination of amino acid substitutions designed to improve spectral properties. The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization. The monomeric Cerulean (mCerulean) is a variant of ECFP (ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization. (J:125961)