Tg(Thy1-Brainbow1.0)HLich

The Thy1-Brainbow 1.0 transgene was designed with the mouse Thy1 regulatory elements surrounding three fluorescent protein (red (RFP), enhanced yellow (EYFP), or cyan (CFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.0 coding region contained (from 5'to 3') a Lox2272 site, LoxP site, tdimer2(12) RFP (with polyA sequence), Lox2272 site, monomeric EYFP (mYFPA206K; with membrane tethering palmitoylation sequence and polyA sequence), LoxP site, mCerulean CFP (with membrane tethering palmitoylation sequence and 5'FRT site and polyA sequence). This transgene was microinjected into C57BL/6JxCBA hybrid oocytes. The tdimer2(12) RFP is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer). The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization. The monomeric Cerulean (mCerulean) is a variant of ECFP (ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization. (J:125961)