An IRES-lacZ and floxed or frt neo cassette were inserted into the 3' end of exon 9. Insertion was confirmed by PCR of genomic DNA. A beta-galactosidase assay was used to confirm expression from the lacZ gene. RT-PCR was used to confirm that endogenous gene expression was unaffected. (J:125817)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
Insertion
--
1
4
5

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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