An 80kb BAC encoding the Spry2 locus was isolated from a CITB-CJ7 (129S1/Sv) BAC library and used for pronuclear injection into C57BL/6J oocytes. Southern blot analysis determined a copy number of 1-2 for line 69. RT-PCR analysis of E14.5 embryonic palate tissue showed that line 69 expressed the transgenic Spry2 at ~25% of wild-type levels. (J:125229)