Log In|Sign Up
EN
A targeting vector was constructed that contains a Sox10 open reading that changes amino acids 71-73 from CIR to AAA, followed by a loxP flanked neo cassette. The region mutated is necessary for DNA-dependent dimerization of this transcription factor. The vector replaced the entire open reading frame of Sox10 by deleting exons 3 through 5. The neo cassette was removed via in vivo Cre mediated recombination. Quantitative RT-PCR and immunoprecipitation on mid-stage embryos indicated that mRNA and protein levels of the mutated gene were similar to the wild-type gene. (J:124679)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
