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To generate the I-Ab-restricted CD4+ 1H3.1 T cell hybridoma, a peptide corresponding to residues 52-68 of the a-chain of the I-E MHC class II molecule (Ea52-68) was injected into C57BL/6 mice. T cells expressing the T cell receptor (TCR) combination (Va1-Ja21 and Vb6-Db2.1-Jb2.6) were selected. These TCR sequences were cloned into TCR expression cassettes and the resulting constructs were co-microinjected into (C57BL/6 x SJL)F2 oocytes. Mature T cells from 1H3.1 TCR Tg mice (Va1Vb6) are skewed toward expression of the CD4 coreceptor. A subset of TCRhigh CD8+ T cells (5-10%) is found in the periphery. (J:124574)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
