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A targeting construct with a long arm for recombination encompassing 4429 bp of Cyp1a2 genomic sequence plus 48 bp of 3'-flanking sequence was followed by a floxed PGK-NEO gene and then by 1452 bp of 3'-flanking sequence for the short arm. An HSK-TK negative-selection cassette was placed outside the 3' Cyp1a2 homology arm. This construct was electroporated into 129S6/SvEvTac ES cells. In recombined ES cells, an allele containing a floxed PGK-NEO gene, placed 350 bp 3' of the stop codon and a loxP site located 48 bp 3' beyond the end of exon 7 was generated. (J:122409)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
