A FRT-flanked neo cassette followed by a loxP site was inserted in the first intron of the targeted gene. An additional loxP site was added downstream of exon 3. Mice were bred to Flpe-expressing mice to excise the neo cassette, leaving exons 2 and 3 flanked by loxP sites. (J:124126)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count