Log In|Sign Up
EN
Using homologous recombination in a bacterial artificial chromosome (BAC, clone RPCI23-331K23), an FRT-flanked neo cassette (followed by a loxP site) was inserted immediately downstream of exon 8 of the targeted gene. An additional loxP site was added upstream of exon 8 when the targeting vector was excised from the BAC. Correctly targeted ES cells were injected into blastocysts and chimeric mice were bred to Flpe-expressing mice to excise the neo cassette. (J:124157)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
