Bsx^tm2Aoh

Targeting took place in PC3 ES cells homozygous for the Tg(Prm-cre)70Og transgene, which expresses Cre recombinase in male germ cells. A targeting vector containing lacZ sequence and a floxed neo cassette was inserted by homologous recombination in ES cells. The neo cassette was subsequently removed upon transmission from male chimeric mice, resulting in the deletion of the homeodomain and expression of the lacZ reporter. Absence of endogenous transcript was confirmed by RT-PCR. (J:123638)