TgTn(pb-ZG-s)1Mrc

Tandem repeats of the small size ZG gene trap construct consisting of piggyBac terminal repeats and internal sequence with all potential splice donor sites removed flanking a loxP site, a splice acceptor site, an IRES-beta-galactosidase-polyA cassette, an FRT site, a CAG-eGFP-polyA cassette, a loxP site, a lox5171 site, and an FRT site. Flp-mediated recombination removes the eGFP cassette. Cre-mediated recombination removes the splice acceptor and beta-galactosidase cassette restoring gene expression. Six female founders were generated and used to create a line. This line was created by co-injection with Tg(Prm1-pb)1Mrc. (J:123007)