The variable exon was replaced with an in-frame eGFP reporter followed by a floxed ACE-cre-neo self-excising cassette. After Cre-mediated excision a single loxP site is left 3' of the eGFP. (J:123007)
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The variable exon was replaced with an in-frame eGFP reporter followed by a floxed ACE-cre-neo self-excising cassette. After Cre-mediated excision a single loxP site is left 3' of the eGFP. (J:123007)