Tg(PDGFB-APPSwInd)J9Lms

The transgene contains a human APP minigene encoding the Indiana mutation (V717F) which is associated with familial Alzheimer disease. The construct contained APP introns 6-8, allowing alternative splicing of exons 7 and 8. The human PDGF beta-chain promoter drives expression, and the construct also contains the SV40 polyadenylation signal. The Swedish APP (K670N/M671L) mutation was introduced into the construct by PCR primer modification. The modified construct was microinjected into (C57BL/6 x DBA/2)F2 one-cell embryos. Mice show high levels of amyloid beta deposits in their hippocampi. (J:23080, J:100974)