The coding sequence was replaced with cDNA encoding the protein Venus, a derivative of GFP, and a neomycin resistance cassette. Absence of endogenous protein was confirmed by immunoblotting of mutant thymus samples. (J:121869)
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The coding sequence was replaced with cDNA encoding the protein Venus, a derivative of GFP, and a neomycin resistance cassette. Absence of endogenous protein was confirmed by immunoblotting of mutant thymus samples. (J:121869)