A loxP site was inserted into intron 1. The wild-type cDNA with a 3xFlag epitope tag-pA cassette was inserted into in exon 3. A neomycin resistance gene was inserted downstream of the cassette and loxP site. (J:122927)
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A loxP site was inserted into intron 1. The wild-type cDNA with a 3xFlag epitope tag-pA cassette was inserted into in exon 3. A neomycin resistance gene was inserted downstream of the cassette and loxP site. (J:122927)