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A 2171-bp SacI/SacII DNA restriction fragment encompassing the 3' end of exon 5 through the 5' end of exon 7 was replaced by an internal ribosomal entry site (IRES) followed by the coding sequence for humanized Renilla green fluorescent protein (hrGFP) and a loxP-flanked neomycin resistance (PGK1-neo) cassette. RT-PCR using primers within the deleted sequence detect no transcript in homozygous mutant lung; using primers from a non-deleted region reveals only trace amounts of mRNA. Absence of the endogenous protein was confirmed by immunohistochemistry of mutant lung tissue. No hrGFP protein is detected by fluorescence confocal microscopy, immunoblot or immuhohistochemical analysis using antibodies to hrGFP. (J:122736)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
