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A targeting vector was designed to fuse a signal peptide from rat neurexin 1 alpha followed by a GFP "emerald" cassette to the amino(N) terminus of exon 1, flank exon 2 with loxP sites, and insert an Flp-flanked neomycin resistance cassette in intron 2. Resultant mice were crossed with an FRT recombinase strain to remove the FLP-flanked neomycin cassette. Presence of the GFP fusion protein was detected by western blot analysis. (J:122459)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
