Gfra1^tm2.1Jmi

A loxP site flanked Gfra1 cDNA (with SV40 poly(A) signal sequences), a GFP gene (with poly(A) signal sequences), and an FRT site flanked neomycin resistance gene cassette were introduced into exon 2. 95 Nucleotides containing the 5' UTR, the initiator ATG codon and the signal sequences, were deleted. The neo cassette was removed through subsequent flp-mediated recombination. The inserted cDNA was deleted through cre-mediated recombination, thus abolishing any Gfra1 expression from this allele, but allowing expression of the GFP gene. (J:122607)