A loxP-flanked transcriptional stop signal in antisense orientation was inserted into intron 6, exon 7 was fused to human WT1 (lysine, threonine, and serine version termed +KTS) with a C-terminal FLAG tag, and a 3' neo cassette, replacing exons 8 through 11, was inserted by homologous recombination. The absence of endogenous protein product was confirmed by western blot analysis. (J:122058)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S6/SvEvTac
Targeted
Insertion, Intragenic deletion
--
1
6
10

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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