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A mouse Plin cDNA that had serine-to-alanine mutations made in all 6 protein kinase A phosphorylation sites using recombinant adenoviruses (Muta-Gene Phagemid in vitro mutagenesis kit (Bio-Rad)). The cDNA was FLAG-tagged at the carboxy terminus via PCR and was placed in a pBluescript vector containing the Fabp2 enhancer/promoter region, the SV40 small tumor antigen splice site, and polyadenylation signal sequence. This construct was injected in C57BL/6 fertilized oocytes. Mice express the transgene almost exclusively in brown adipose tissue. (J:113735)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
