A loxP site, and a frt-flanked neomycin resistance cassette was inserted upstream of exon 2, and another loxP site and an EcoRI restriction site was inserted 1 kb downstream of exon 2. Correct targeting was confirmed by Southern blotting and genomic PCR. (J:139846)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count