A targeting vector was designed to replace the coding region in exon 1 and 2 with GFP. RT-PCR confirmed the absence of transcript in mutants. A floxed neo included in the vector was subsequently removed via cre mediated recombination. (J:121899)
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A targeting vector was designed to replace the coding region in exon 1 and 2 with GFP. RT-PCR confirmed the absence of transcript in mutants. A floxed neo included in the vector was subsequently removed via cre mediated recombination. (J:121899)