An FRT-flanked neo was removed via Flp mediated recombination, leaving point mutations in the E2F site and the non-coding sequence of exon 1. Expression of transcript was as high, if not higher, than wild-type. (J:121059)
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An FRT-flanked neo was removed via Flp mediated recombination, leaving point mutations in the E2F site and the non-coding sequence of exon 1. Expression of transcript was as high, if not higher, than wild-type. (J:121059)