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A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence in-frame with the ATG transcriptional start site of the mouse pro-opiomelanocortin-alpha (Pomc1) gene (this also deleted the first 30 nucleotides of Pomc1). Correctly transformed and targeted bacteria were used to isolate the 150 kb BAC, which was then injected into the pronucleus of fertilized one-cell stage FVB embryos. Transgenic line 1 was subsequently established. When exposed to UV light, brain tissue shows GFP fluorescence patterns consistent with endogenous Pomc1 expression. (J:120992, J:125165)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
