Tg(CD2-TcraF5,CD2-TcrbF5)1Kio

Constructs were created, with the cDNAs for both the alpha chain (Valpha4) and the beta chain (Vbeta11) of the T cell receptor from the mouse CTL F5 clone, inserted in the 5' UTR of the human CD2 minigene carrying a frameshift mutation in its coding region. The CD2 minigene provides an intron, the 3' LCR and a polyadenylation signal which are sufficient to direct expression of the transgenes. The constructs were coinjected into C57BL/10 oocytes. Transmission of the transgenes produced several lines, and Southern blot verified multiple copies of the alpha and beta chain genes in one line. The transgene is specific for specific for influenza virus NP peptide presented by H-2Db. (J:113713)