LoxP sites were inserted to flank exon 2, as well as tk and neo selection markers. The selection markers were removed by transient transfection with Cre recombinase, leaving a floxed exon 2. (J:119977)
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LoxP sites were inserted to flank exon 2, as well as tk and neo selection markers. The selection markers were removed by transient transfection with Cre recombinase, leaving a floxed exon 2. (J:119977)