Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe

A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), human AML1-ETO fusion protein sequence (coding for the translocation t(8;21) present in 15% of acute myeloid leukemias (AML)), internal ribosomal entry site, and the human Placental Alkaline Phosphatase (ALPP or hPLAP) gene. Expression of lacZ is observed in all tissues including bone marrow progenitor cells. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human AML1-ETO fusion protein and placental alkaline phosphatase (ALPP or PLAP) to proceed in the Cre-expressing cells. (J:119131)