Lhx6^tm2Vpa

Exons 2 through 4 and part of exon 5 were replaced with an IRES-eGFP and a floxed PGK-neo cassette. The neo cassette was then removed in vivo by Cre-mediated recombination. Immunohistochemistry and western blot analysis confirmed near total loss of protein expression in homozygous mice. No GFP expression is seen in heterozygotes. (J:119476)